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shRNA Knockdown服務

>>服務特色

  • 擔保設計的shRNA對靶基因的Knockdown效率>60%

  • 多樣化篩選標記(puromycin、GFP、RFP)

  • 免費提供標準的scrambled control

  • 價格合理,病毒載體免費使用

  • 一站式服務,基因合成、載體構建、病毒包裝、細胞克隆篩選驗證一步到位

>>技術流程

shRNA 慢病毒 載體構建 靶細胞感染 FACS WesternBlot測定 Q-PCR Sanger測序 慢病毒滴度測定 QC.jpg

  • shRNA載體構建

  • 慢病毒制備

  • 慢病毒滴度測定

  • 慢病毒感染靶細胞

  • qPCR驗證knockdown效率

  • 周期:6-8周


  • 目的基因信息

  • 靶細胞及培養條件

  • 重組細胞株驗證方法

  • 實驗報告

  • 凍存穩定細胞株2管(>1*10^6細胞/管)

0086-512-62570431

[email protected]

时时彩名爵平台
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